Windows:
Yes. iTunes will need to convert WMA files to AAC / MP3 format first though:
Simply drag the WMA file(s) into your iTunes library, and the files will be converted for you – you can then play the songs in iTunes and listen to them on your iPod.
* Windows Media Player 9 or later must be installed to convert WMA files.
Mac:
Unfortunately WMA files are not currently supported by iTunes for Mac. We hope to offer a fully Mac-compatible solution soon.
Mac users must install Windows Media Player for Mac in order to play WMA files. There are also some alternative players available for free:
You can convert WMA files to iTunes-compatible MP3 files by using Easy WMA - please note that this software is not free, and costs $10 USD at the time of writing.
Please note that Musicroom.com is not responsible for (and does not vouch for the quality of) the content at the external links above.